Human immunoglobulin AFc segment receptor I (FcαRI/CD89) ELISA kit instruction manual - Database & Sql Blog Articles

Welcome to our official website! As a leading supplier of scientific research reagents in China, we specialize in the distribution of imported sub-packaged and original products, as well as domestic ELISA kits. We guarantee high-quality standards, advanced technology, and offer a no-effect refund policy. For inquiries or orders, please contact us at 60536566. This kit is intended for research use only and is not suitable for medical diagnosis. It is designed for the quantitative detection of Human Immunoglobulin Fc Receptor I (FcαRI/CD89) in serum, plasma, and related fluid samples. **Kit Name:** Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit **Application:** Quantitative detection of FcαRI/CD89 in human samples **Principle:** The kit uses a two-antibody sandwich ELISA method. After incubation, unbound components are washed away. A TMB substrate is added, and the reaction is stopped with an acidic solution. The color change is measured at 450 nm, and the concentration is determined by comparing the OD values of the standard and the sample. **Kit Components:** - Enzyme-labeled plate (12×8) - Substance A (6 mL) - Standard (200 ng/mL, 0.6 mL) - Substrate B (6 mL) - Wash solution (20×, 20 mL) - Sample diluent (6 mL) - Enzyme standard reagent (6 mL) - Stop solution (6 mL) - Instruction manual - Sealing film (1 sheet) - Sealed bag (1 piece) **Preparation and Usage Notes:** - Standard solutions should be diluted to concentrations of 200, 100, 50, 25, 12.5, and 6.25 ng/mL. - Required equipment includes a 37°C incubator, microplate reader, pipettes, distilled water, test tubes, and absorbent paper. **Steps:** 1. Bring the kit to room temperature for 30 minutes. 2. Dilute the washing solution with distilled water. 3. Add standards and samples to the plate, including blank control wells. 4. Incubate at 37°C for 30 minutes. 5. Wash the plate 4 times. 6. Add enzyme working solution to each well. 7. Incubate again for 30 minutes. 8. Repeat washing steps. 9. Add TMB substrates A and B, and develop the color for 15 minutes in the dark. 10. Stop the reaction with stop solution. 11. Measure OD values at 450 nm within 15 minutes. 12. Calculate results using a standard curve. **Sample Requirements:** - Avoid sodium azide contamination. - Store samples at -20°C if not tested immediately. - Ensure samples are centrifuged and free from hemolysis and particles. **Precautions:** - Follow instructions strictly. - Store unused enzyme labels properly. - Perform duplicate testing for accuracy. - Account for dilution factors when calculating final concentrations. - Avoid cross-contamination between samples and reagents. - Do not mix reagents from different batches. - Protect substrate B from light exposure. **Operating Summary:** - Prepare all reagents, samples, and standards. - Add samples and standards to the plate, incubate at 37°C for 30 minutes. - Wash the plate four times. - Add enzyme working solution and incubate again. - Add TMB substrates and develop color. - Stop the reaction and measure OD values. - Calculate and interpret results based on the standard curve. **Detection Range:** 0.1–200 ng/mL **Specifications:** 96T/box, 48T/box **Storage Conditions:** 2–8°C, protected from light and moisture **Shelf Life:** 6 months If you have any questions about the kit or need assistance, feel free to reach out. We’re here to help!

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