Human immunoglobulin AFc segment receptor I (FcαRI/CD89) ELISA kit instruction manual - Database & Sql Blog Articles

As a leading domestic supplier of scientific research reagents, we specialize in imported sub-packaged and original products, as well as domestic ELISA kits. We guarantee quality and advanced technology, offering a no-effect refund policy. Feel free to contact us for inquiries or orders at 60536566. Kit Can only be used for scientific research Not for medical diagnosis Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit User's Guide 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Kit Name: Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Kit Use: Quantitative detection of Immunoglobulin Fc segment receptor I (FcαRI/CD89) in serum, plasma, and related fluid samples. 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Detection Principle: The kit uses a two-antibody sandwich ELISA method. Standards and samples are added to the pre-coated enzyme-labeled plate. After incubation, unbound components are washed away. Substrates A and B are then added sequentially. TMB is converted to a blue product under horseradish peroxidase (HRP) catalysis, turning yellow when acid is added. The color intensity is directly proportional to the concentration of Immunoglobulin Fc segment receptor I (FcαRI/CD89). OD values are measured at 450 nm, and sample concentrations are calculated based on standard curves. 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Kit Composition: 1. Enzyme-labeled coated plate – 12 wells × 8 strips 2. Substance A – 6 mL 3. Standard – 200 ng/mL – 0.6 mL 4. Substrate B – 6 mL 5. Concentrated washing solution – 20 mL 6. Standard dilution – 6 mL 7. Enzyme standard reagent – 6 mL 8. Stop solution – 6 mL 9. Diluent – 6 mL 10. Instruction manual – 1 copy 11. Sealing film – 1 sheet 12. Sealed bag – 1 bag Notes: Standard solutions should be diluted to 200, 100, 50, 25, 12.5, and 6.25 ng/mL. 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Reagents and Equipment Needed but Not Provided: 1. 37°C incubator 2. Microplate reader 3. Precision pipettes and disposable tips 4. Distilled water 5. Disposable test tubes 6. Absorbent paper 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Steps: 1. Preparation: Remove the kit from the refrigerator and let it reach room temperature for 30 minutes. 2. Dilute the 20× concentrated washing solution with distilled water. 3. Add standards and samples to the plate. Set up standard wells, sample wells, and blank control wells. Add 50 μL of standard to each standard well, 10 μL of sample and 40 μL of diluent to each sample well, and no liquid to the blank control. 4. Incubate at 37°C for 30 minutes. 5. Wash the plate 4 times using the washing solution. 6. Add 50 μL of enzyme standard working solution to each well except the blank control. 7. Incubate again for 30 minutes. 8. Repeat the washing process. 9. Add 50 μL of developer A, then 50 μL of developer B. Incubate in the dark at 37°C for 15 minutes. 10. Add 50 μL of stop solution to each well. 11. Measure OD values at 450 nm within 15 minutes after stopping the reaction. 12. Calculate the sample concentration using the standard curve and regression equation. Multiply by the dilution factor for accurate results. 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Sample Requirements: 1. Avoid sodium azide (NaN₃), which inhibits HRP activity. 2. Samples should be processed and tested as soon as possible. If not tested immediately, store at -20°C and avoid repeated freezing and thawing. 3. Ensure samples are fully centrifuged and free of hemolysis and particles. 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Precautions: 1. Follow the manual strictly and base results on microplate reader readings. 2. Store unused enzyme labels in a sealed bag with desiccant. 3. Perform double testing for all standards, samples, and blanks. 4. Remember that the sample was diluted 5 times; multiply the result by 5 for actual concentration. 5. The detection range is 0.1–200 ng/mL. If outside this range, dilute appropriately and recalculate. 6. If the color is too light, extend the substrate incubation time slightly. 7. Prevent cross-contamination by using fresh materials for each addition. 8. Do not mix reagents from different batches. 9. Protect Substrate B from light exposure. 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Summary of Operating Procedures: - Prepare reagents, samples, and standards - Add samples and standards, incubate at 37°C for 30 min - Wash the plate 4 times - Add enzyme standard reagent, incubate for 30 min - Add substrates A and B, develop color for 15 min - Add stop solution - Read OD values within 15 min and calculate results 【Human Immunoglobulin Fc Receptor I (FcαRI/CD89) ELISA Kit】 Testing Range: 0.1–200 ng/mL Specification: 96T/box, 48T/box Storage: 2–8°C, protected from light and moisture Validity Period: 6 months

Double Convex Cylindrical Lens

Cylindrical lenses are typically applied to focus incoming light to a line, or to change the aspect ratio of an image. they are used for the optics, lasers, medical science, electronics, tele-communication and others.
Realpoo Optics can offer cylindrical Lenses include convex Cylindrical Lens, concave cylindrical lens, meniscus cylindrical lens, and doublet achromatic cylindrical lens ect.


Specifications:

Material : N-BK7, N-SF1 , Fused Silica , N-SF10 etc
Diameter : 2mm ~ 300mm
Focal Legnth Tolerance :+/-1%
Diameter Tolerance :+0.0/-0.1mm
Thickness Tolerance :+/-0.1mm
Surface Quality :40/20 or better
Centration :<3 arc minutes
Clear Aperture :>90%
Bevel : Protective bevel as needed
Coating :Upon your request

Double Convex Cylindrical Lens,Cylindrical Lens,Laser Line Generator Optics,Laser Line Generator Lens

Changchun Realpoo Photoelectric Co., Ltd. , https://www.optics-realpoo.com