Shanghai Yuanmu detailed: ELISA kit experimental preparation 6 elements! -Huaqiang Electronic Network

EL-C1600N100013-B
Spot Remarks 2017+
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Kaixin micro test
Shanghai Yuanmu Tips: 6 Essential Steps for Preparing Your ELISA Kit! Proper preparation is the foundation of a successful ELISA experiment. Even small mistakes can lead to inaccurate results, so it's essential to follow best practices. Here are six key elements you should pay attention to before starting your experiment. 1. **Let the kit come to room temperature** – Take the ELISA kit out of the refrigerator at least one hour before the experiment begins. This helps ensure that all reagents reach a stable temperature, which improves the reliability of your results. 2. **Keep the substrate in the dark** – Some substrates used in ELISA are light-sensitive. Store them away from direct light during the experiment to prevent degradation and maintain accuracy. 3. **Avoid air bubbles when pipetting** – When using a pipette, do not draw liquid too quickly. Moving too fast can introduce air bubbles, which may affect the volume and accuracy of your measurements. 4. **Use the correct pipette size** – Always choose a pipette that closely matches the volume you need to measure. This reduces error and ensures more precise results. 5. **Check pipette accuracy regularly** – The accuracy of your pipette is crucial. You can test it using water and an electronic balance. If the error exceeds 2%, consider having it calibrated by a professional. 6. **Use multiple pipettes for different volumes** – It’s recommended to have a set of pipettes for 20 µL, 50 µL, 100 µL, and 1000 µL. Replace the tips after each use, even when handling standards, to avoid cross-contamination. Our ELISA kits are widely used in protein detection due to their high sensitivity and ease of use. Available for species including human, rat, mouse, cow, sheep, horse, rabbit, chicken, fish, duck, and more. Contact us today to find the right kit for your research needs!

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