Basic conditions for the correct cultivation of cells - Shanghai Jinma Bio-华强电子网

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Cell culture is a technique used to grow and maintain cells outside their natural environment. By isolating cells from tissues in vivo, scientists can mimic the conditions found inside the body, allowing cells to grow, reproduce, and retain their original structure and function. This process can involve either a single cell or a population of cells.

1. Suitable Cell Culture Medium

A suitable cell culture medium is one of the most critical factors for successful in vitro cell growth. It provides essential nutrients, supports cell proliferation, and creates an optimal environment for cellular activity. The right medium helps maintain cell viability and functionality over time.

2. High-Quality Serum

Most synthetic media require the addition of serum, which is a vital component in cell culture. Serum contains various growth factors, hormones, and other nutrients necessary for cell survival and development. It also helps regulate pH and provides a stable environment for cell growth.

3. Sterile and Non-Toxic Environment

Creating a sterile and non-toxic environment is crucial for successful cell culture. In vitro cultured cells are highly sensitive to contamination, as they lack the immune defenses present in living organisms. Even minor exposure to pathogens or toxic substances can lead to cell death. Therefore, maintaining a clean and controlled environment is essential to ensure cell health and longevity.

4. Constant Temperature

Cells require a stable and appropriate temperature to thrive. Fluctuations in temperature can disrupt metabolic processes and affect cell growth. Most mammalian cells grow best at 37°C, simulating the human body's internal conditions.

5. Proper Gas Environment

The gas composition in the culture environment plays a key role in cell survival, especially for mammalian cells. Oxygen and carbon dioxide are the two main gases required. Oxygen supports cellular respiration, while carbon dioxide helps maintain the pH balance of the culture medium.

Shanghai Jinma Bio-Agent offers a wide range of biological products, including cell lines, culture media, and related reagents. Whether you're conducting research or industrial cell culture, we provide high-quality solutions tailored to your needs. Feel free to contact us for more information!